期刊
DEVELOPMENT
卷 138, 期 17, 页码 3723-3733出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.063362
关键词
GnRH neuron; Neuronal survival; VEGF; KDR; VEGFR2; FLK1; Neuropilin; Mouse
资金
- BBSRC [BB/F009658/1]
- Wellcome Trust [089775/Z/09/Z]
- Wellcome Trust [089775/Z/09/Z] Funding Source: Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/F009658/1] Funding Source: researchfish
- BBSRC [BB/F009658/1] Funding Source: UKRI
Gonadotropin-releasing hormone (GnRH) neurons are neuroendocrine cells that are born in the nasal placode during embryonic development and migrate through the nose and forebrain to the hypothalamus, where they regulate reproduction. Many molecular pathways that guide their migration have been identified, but little is known about the factors that control the survival of the migrating GnRH neurons as they negotiate different environments. We previously reported that the class 3 semaphorin SEMA3A signals through its neuropilin receptors, NRP1 and NRP2, to organise the axons that guide migrating GnRH neurons from their birthplace into the brain. By combining analysis of genetically altered mice with in vitro models, we show here that the alternative neuropilin ligand VEGF164 promotes the survival of migrating GnRH neurons by co-activating the ERK and AKT signalling pathways through NRP1. We also demonstrate that survival signalling relies on neuronal, but not endothelial, NRP1 expression and that it occurs independently of KDR, the main VEGF receptor in blood vessels. Therefore, VEGF164 provides survival signals directly to developing GnRH neurons, independently of its role in blood vessels. Finally, we show that the VEGF164-mediated neuronal survival and SEMA3A-mediated axon guidance cooperate to ensure that migrating GnRH neurons reach the brain. Thus, the loss of both neuropilin ligands leads to an almost complete failure to establish the GnRH neuron system.
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