期刊
DEVELOPMENT
卷 138, 期 11, 页码 2171-2183出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.056507
关键词
bHLH; Dopamine; Drosophila; Lethal of scute; Neural cell fate; Tailup
资金
- NIH [R37 RD25251 (NICHD), R01 NS64264 (NINDS)]
- NIH/UNC
- UNC Developmental Biology NIH
Dopaminergic neurons play important behavioral roles in locomotion, reward and aggression. The Drosophila H-cell is a dopaminergic neuron that resides at the midline of the ventral nerve cord. Both the H-cell and the glutamatergic H-cell sib are the asymmetric progeny of the MP3 midline precursor cell. H-cell sib cell fate is dependent on Notch signaling, whereas H-cell fate is Notch independent. Genetic analysis of genes that could potentially regulate H-cell fate revealed that the lethal of scute [l(1)sc], tailup and SoxNeuro transcription factor genes act together to control H-cell gene expression. The l(1) sc bHLH gene is required for all H-cell-specific gene transcription, whereas tailup acts in parallel to l(1) sc and controls genes involved in dopamine metabolism. SoxNeuro functions downstream of l(1) sc and controls expression of a peptide neurotransmitter receptor gene. The role of l(1) sc may be more widespread, as a l(1) sc mutant shows reductions in gene expression in non-midline dopaminergic neurons. In addition, l(1) sc mutant embryos possess defects in the formation of MP4-6 midline precursor and the median neuroblast stem cell, revealing a proneural role for l(1) sc in midline cells. The Notch-dependent progeny of MP4-6 are the mVUM motoneurons, and these cells also require l(1) sc for mVUM-specific gene expression. Thus, l(1) sc plays an important regulatory role in both neurogenesis and specifying dopaminergic neuron and motoneuron identities.
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