4.7 Article

FatJ acts via the Hippo mediator Yap1 to restrict the size of neural progenitor cell pools

期刊

DEVELOPMENT
卷 138, 期 10, 页码 1893-1902

出版社

COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.064204

关键词

Cadherin; Neural tube; Chick; RNAi; Screen; FatJ; Progenitor cells; Interneurons; Phenotype; Hippo pathway; Notch signalling; Morphogen

资金

  1. BBSRC
  2. Wellcome grant
  3. MRC
  4. Wellcome Trust [GR077544AIA]
  5. MRC [G0500826, G0501618] Funding Source: UKRI
  6. Biotechnology and Biological Sciences Research Council [BBS/B/13578] Funding Source: researchfish
  7. Medical Research Council [G0501618, G0500826] Funding Source: researchfish

向作者/读者索取更多资源

The size, composition and functioning of the spinal cord is likely to depend on appropriate numbers of progenitor and differentiated cells of a particular class, but little is known about how cell numbers are controlled in specific cell cohorts along the dorsoventral axis of the neural tube. Here, we show that FatJ cadherin, identified in a large-scale RNA interference (RNAi) screen of cadherin genes expressed in the neural tube, is localised to progenitors in intermediate regions of the neural tube. Loss of function of FatJ promotes an increase in dp4-vp1 progenitors and a concomitant increase in differentiated Lim1(+)/Lim2(+) neurons. Our studies reveal that FatJ mediates its action via the Hippo pathway mediator Yap1: loss of downstream Hippo components can rescue the defect caused by loss of FatJ. Together, our data demonstrate that RNAi screens are feasible in the chick embryonic neural tube, and show that FatJ acts through the Hippo pathway to regulate cell numbers in specific subsets of neural progenitor pools and their differentiated progeny.

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