期刊
DEVELOPMENT
卷 138, 期 24, 页码 5393-5402出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.068452
关键词
Foetal ovary; Germ cells; Msx; Mouse; Human
资金
- Universite Paris Diderot-Paris
- Commissariat a l'Energie Atomique (CEA)
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Agence Francaise de Securite Sanitaire de l'Environnement et du Travail (AFFSET)
- Electricite de France (EDF)
- Agence Nationale de la Recherche [ANR] [1239 03]
The mechanisms regulating germ line sex determination and meiosis initiation are poorly understood. Here, we provide evidence for the involvement of homeobox Msx transcription factors in foetal meiosis initiation in mammalian germ cells. Upon meiosis initiation, Msx1 and Msx2 genes are strongly expressed in the foetal ovary, possibly stimulated by soluble factors found there: bone morphogenetic proteins Bmp2 and Bmp4, and retinoic acid. Analysis of Msx1/Msx2 double mutant embryos revealed a majority of undifferentiated germ cells remaining in the ovary and, importantly, a decrease in the number of meiotic cells. In vivo, the Msx1/Msx2 double-null mutation prevented full activation of Stra8, a gene required for meiosis. In F9 cells, Msx1 can bind to Stra8 regulatory sequences and Msx1 overexpression stimulates Stra8 transcription. Collectively, our data demonstrate for the first time that some homeobox genes are required for meiosis initiation in the female germ line.
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