期刊
DEVELOPMENT
卷 136, 期 5, 页码 729-738出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.025569
关键词
Evolution; Kaiso; MBT; Siamois; Methyl-CpG binding; Xenopus laevis
资金
- Russian Academy of Sciences program grant Molecular and Cellular Biology [RFBR 06-04-49216-a, CRDF RB-2808]
- [CR10-A-04]
- [08410708700]
- BBSRC [BB/E023355/1] Funding Source: UKRI
- MRC [MC_U127574433] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E023355/1] Funding Source: researchfish
- Medical Research Council [MC_U127574433] Funding Source: researchfish
Mammalian forms of the transcription repressor, Kaiso, can reportedly bind methylated DNA and non-methylated CTGCNA motifs. Here we compare the DNA-binding properties of Kaiso from frog, fish and chicken and demonstrate that only the methyl-CpG-binding function of Kaiso is evolutionarily conserved. We present several independent experimental lines of evidence that the phenotypic abnormalities associated with xKaiso-depleted Xenopus laevis embryos are independent of the putative CTGCNA-dependent DNA-binding function of xKaiso. Our analysis suggests that xKaiso does not play a role in the regulation of either xWnt11 or Siamois, key signalling molecules in the Wnt pathway during X. laevis gastrulation. The major phenotypic defects associated with xKaiso depletion are premature transcription activation before the mid-blastula transition and concomitant activation of a p53-dependent cell-death pathway.
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