期刊
DEVELOPMENT
卷 136, 期 2, 页码 253-262出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.029900
关键词
GABA; Interneuron; Midbrain; Neurotransmitter; Mouse; Neurogenesis; Ventral tegmental area (VTA); Serotonin; Dorsal raphe; Rhombomere 1
资金
- Marie Curie ESTP (M. H.), Academy of Finland
- Biocentrum Helsinki and Sigrid Juselius Foundation
Midbrain GABAergic neurons control several aspects of behavior, but regulation of their development and diversity is poorly understood. Here, we further refine the midbrain regions active in GABAergic neurogenesis and show their correlation with the expression of the transcription factor Gata2. Using tissue-specific inactivation and ectopic expression, we show that Gata2 regulates GABAergic neuron development in the mouse midbrain, but not in rhombomere 1, where it is needed in the serotonergic lineage. Without Gata2, all the precursors in the embryonic midbrain fail to activate GABAergic neuron-specific gene expression and instead switch to a glutamatergic phenotype. Surprisingly, this fate switch is also observed throughout the neonatal midbrain, except for the GABAergic neurons located in the ventral dopaminergic nuclei, suggesting a distinct developmental pathway for these neurons. These studies identify Gata2 as an essential post-mitotic selector gene of the GABAergic neurotransmitter identity and demonstrate developmental heterogeneity of GABAergic neurons in the midbrain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据