期刊
ACS NANO
卷 9, 期 6, 页码 6532-6547出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b02483
关键词
chemical origin; nanotoxicity; dynamic processes of intracellular nanoparticles; chemical transformation; AgNPs; nano-CT
类别
资金
- MOST 973 program [2011CB933400]
- International Science AMP
- Technology Cooperation Program of China [2013DFG32340, 2014DFG52500]
- NSFC [21320102003, 21277037, 11205166, 11435002]
- National Science Fund for Distinguished Young Scholars [11425520]
- Major Equipment Program [2011YQ030134]
- Beijing NSF [2152037]
To predict potential medical value or toxicity of nanoparticles (NPs), it is necessary to understand the chemical transformation during intracellular processes of NPs. However, it is a grand challenge to capture a high-resolution image of metallic NPs in a single cell and the chemical information on intracellular NPs. Here, by integrating synchrotron radiation-beam transmission X-ray microscopy (SR-TXM) and SR-X-ray absorption near edge structure (SR-XANES) spectroscopy, we successfully capture the 3D distribution of silver NPs (AgNPs) inside a single human monocyte (THP-1), associated with the chemical transformation of silver. The results reveal that the cytotoxicity of AgNPs is largely due to the chemical transformation of particulate silver from elemental silver (Ag-0)(n), to Ag+ ions and Ag-0-, then Ag-S- species. These results provide direct evidence in the long-lasting debate on whether the nanoscale or the ionic form dominates the cytotoxicity of silver nanoparticles. Further, the present approach provides an integrated strategy capable of exploring the chemical origins of cytotoxicity in metallic nanoparticles.
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