期刊
DENTAL MATERIALS
卷 28, 期 12, 页码 1250-1260出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dental.2012.09.002
关键词
Biomaterials; Sol-gel; Soft-lithography; Micropatterning
资金
- FEDER funds through the Programa Operacional Factores de Competitividade - COMPETE
- Portuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia [FCT/SFRH/BD/36220/2007, FCT/PTDC/CTM/100120/2008]
- CRUP - Accoes integradas Luso-Espanholas [E46/09]
- Acciones integradas Hispano Portuguesas
- Ministry of Science, Innovation of Spain [MAT2010-17753]
- NSEC (NSF Nanoscale Science and Engineering Center, USA)
- [MICINN: HP2008-0075]
- Directorate For Engineering
- Div Of Engineering Education and Centers [914790] Funding Source: National Science Foundation
Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective. The main goal of this study was to produce micro-fabricated SiO2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods. A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results. Micropatterned SiO2 surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance. The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. (C) 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
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