期刊
DENTAL MATERIALS
卷 24, 期 9, 页码 1212-1222出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dental.2008.02.001
关键词
calcium phosphate cement; nano-apatite; macroporous scaffolds; stress-bearing; osteoblast cell delivery; growth factor; bone tissue engineering
资金
- NIDCR NIH HHS [R01 DE14190, R01 DE014190-01A2, R01 DE014190-06, R01 DE014190] Funding Source: Medline
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE014190] Funding Source: NIH RePORTER
Objectives. Seven million people suffer bone fractures each year in the U.S., and musculoskeletal conditions cost $215billion/year. The objectives of this study were to develop moldable/injectable, mechanically strong and in situ-hardening calcium phosphate cement (CPC) composite scaffolds for bone regeneration and delivery of osteogenic cells and growth factors. Methods. Tetracalcium phosphate [TTCP: Ca-4(PO4)(2)O] and dicalcium phosphate (DCPA: CaHPO4) were used to fabricate self-setting calcium phosphate cement. Strong and macroporous scaffolds were developed via absorbable fibers, biopolymer chitosan, and mannitol porogen. Following established protocols, MC3T3-E1 osteoblast-like cells (Riken, Hirosaka, Japan) were cultured on the specimens and inside the CPC composite paste carrier. Results. The scaffold strength was more than doubled via reinforcement (p < 0.05). Relationships and predictive models were established between matrix properties, fibers, porosity, and overall composite properties. The cement injectability was increased from about 60% to nearly 100%. Cell attachment and proliferation on the new composite matched those of biocompatible controls. Cells were able to infiltrate into the macropores and anchor to the bone mineral-like nano-apatite crystals. For cell delivery, alginate hydrogel beads protected cells during cement mixing and setting, yielding cell viability measured via the Wst-1 assay that matched the control without CPC (p > 0.1). For growth factor delivery, CPC powder:liquid ratio and chitosan content provided the means to tailor the rate of protein release from CPC carrier. Significance. New CPC scaffolds were developed that were strong, tough, macroporous and osteoconductive. They showed promise for injection in minimally invasive surgeries, and in delivering osteogenic cells and osteoinductive growth factors to promote bone regeneration. Potential applications include various dental, craniofacial, and orthopedic reconstructions (c) 2008 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据