期刊
JOURNAL OF NUCLEAR MEDICINE
卷 56, 期 5, 页码 745-751出版社
SOC NUCLEAR MEDICINE INC
DOI: 10.2967/jnumed.114.152496
关键词
claudin-4; cCPE; SPECT; tumorigenesis; breast cancer
资金
- Cancer Research UK
- CR-UK/EPSRC Cancer Imaging Centre in Oxford
- Max Eder fellowship from the Deutsche Krebshilfe
- Cancer Research UK [16466] Funding Source: researchfish
- Pancreatic Cancer UK [RIF2014_02_Cornelissen] Funding Source: researchfish
The expression of claudin-4, a protein involved in tight junction complexes, is widely dysregulated in epithelial malignancies. Claudin-4 is overexpressed in several premalignant precursor lesions, including those of cancers of the breast, pancreas, and prostate, and is associated with poor survival. A noncytotoxic C-terminal fragment of Clostridium perfringens enterotoxin (cCPE) is a natural ligand for claudin-4. Here, we demonstrate wholebody quantitative SPECT imaging of preneoplastic breast cancer tissue using In-111-labeled cCPE. Methods: cCPE.GST or GST (GST is glutathione S-transferase) was conjugated to the metal ion chelator benzyl-diethylenetriaminepentaacetic acid to allow In-111 radiolabeling. The affinity of radiolabeled cCPE. GST for claudin-4 was confirmed using claudin-4-expressing MDA-MB-468 and SQ20b cells, compared with claudin-4-negative HT1080 cells. In vivo SPECT imaging was performed using athymic mice bearing MDA-MB-468 or HT1080 xenografts and using genetically modified BALB/neuT mice, which spontaneously develop claudin-4-expressing breast cancer lesions. Results: The uptake of In-111-cCPE. GST in claudin-4-positive MDA-MB-468 xenograft tumors in athymic mice was significantly higher than in 111In-GST or claudin-4-negative HT1080 tumors (6.72 +/- 0.18 vs. 3.88 +/- 1.00 vs. 2.36 +/- 1.25 percentage injected dose per gram [% ID/g]; P < 0.0001). No other significant differences were observed in any of the examined organs. BALB/neuT mice, expressing rat neuT under mmtv promotor control, spontaneously developed tumorous lesions within their mammary fat pads over the course of 130 d. Overt mammary tumors were claudin-4-positive, and In-111-cCPE. GST uptake was 3.2 +/- 0.70 % ID/g, significantly higher than In-111-GST (1.00 +/- 0.60 % ID/g; P < 0.05). Mammary fat pads in mice aged 80 d bore claudin-4-positive aplastic lesions and accumulated In-111-cCPE. GST (3.17 +/- 0.51 % ID/g) but not In-111-GST (0.99 +/- 0.39 % ID/g; P < 0.001). Conclusion: Taken together, In-111-cCPE. GST targets claudin-4 expression in frank tumors and preneoplastic tissue, and cCPE imaging may be used as an early detection tool for breast, prostate, and pancreatic cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据