期刊
CYTOTHERAPY
卷 16, 期 2, 页码 191-202出版社
INFORMA HEALTHCARE
DOI: 10.1016/j.jcyt.2013.11.008
关键词
cytokines; human; mesenchymal stromal cells; T cells
类别
资金
- Canadian Institutes of Health Research-CIHR [MOP-15017]
Background aims. Mesenchymal stromal cells (MSCs) suppress T-cell proliferation, especially after activation with inflammatory cytokines. We compared the dynamic action of unprimed and interferon (IFN)-gamma plus tumor necrosis factor (TNF)-alpha-pretreated human bone marrow-derived MSCs on resting or activated T cells. Methods. MSCs were co-cultured with allogeneic peripheral blood mononuclear cells (PBMCs) at high MSC-to-PBMC ratios in the absence or presence of concomitant CD3/CD28-induced T-cell activation. The kinetic effects of MSCs on cytokine production and T-cell proliferation, cell cycle and apoptosis were assessed. Results. Unprimed MSCs increased the early production of IFN-gamma and interleukin (IL)-2 by CD3/CD28-activated PBMCs before suppressing T-cell proliferation. In non-activated PBMC co-cultures, low levels of IL-2 and IL-10 synthesis were observed with MSCs in addition to low levels of CD69 expression by T cells and no T-cell proliferation. MSCs also decreased apoptosis in resting and activated T cells and inhibited the transition of these cells into the sub-G0/G1 and the S phases. With inhibition of indoleamine 2,3 dioxygenase, MSCs increased CD3/CD28-induced T-cell proliferation. After priming with IFN-gamma plus TNF-alpha, MSCs were less potent at increasing cytokine production by CD3/CD28-activated PBMCs and more effective at inhibiting T-cell proliferation but had preserved anti-apoptotic functions. Conclusions. Unprimed MSCs induce a transient increase in IFN-gamma and IL-2 synthesis by activated T cells. Pre-treatment of MSCs with IFN-gamma plus TNF-alpha may increase their effectiveness and safety in vivo.
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