期刊
CYTOTHERAPY
卷 16, 期 9, 页码 1220-1228出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jcyt.2014.05.021
关键词
adipose tissue; adult stem cell; density gradient centrifugation; Ficoll; mesenchymal stromal cell; red blood cell lysis; stromal vascular fraction
类别
资金
- Le Fonds National de la Recherche Scientifique of Belgium (FNRS) [7.4.517.09]
- Fund for Scientific Research in Flanders (FWO) [FWOKN259, FWOTM647]
- Research Council of the Vrije Universiteit Brussel
- European Community's Seventh Framework Programme (FP7) (ESNATS) [HEALTH-F5-2008-201619]
- Brussels Institute for Research and Innovation (INNOVIRIS) (Brustem) [IP-LS-7]
- Interuniversity Attraction Pole programme of the Belgian Science Policy Office (IAP-HEPRO BELSPO) [P7/47]
Background aims. Adult human subcutaneous adipose tissue harbors a multipotent stem cell population, the so-called human adipose tissue derived mesenchymal stromal cells (AT-MSCs). These cells are able to differentiate in vitro into various cell types and possess immunomodulatory features. Yet procedures to obtain AT-MSCs can vary significantly. The two most extensively used AT-MSC purification techniques are (i) density gradient centrifugation using Ficoll and (ii) red blood cell (RBC) lysis buffer treatment of the stromal vascular fraction. In the context of potential clinical cell therapy, the stem cell yield after purification and upon consecutive passages, as well as the purity of the obtained cell population, are of utmost importance. Methods. We investigated the expansion capacity and purity of AT-MSCs purified by both procedures immediately after isolation and upon consecutive passages. We also investigated possible purification-dependent differences in their expression of immune-inhibitory factors and cell adhesion molecules. Results. We found that RBC lysis buffer treatment is a more robust and easier method to purify AT-MSCs than density gradient ftactionation. However, the resulting AT-MSC-RBC population contains a significantly higher number of CD34(+) cells, particularly during the first passages after plating. From passage 4 onward, no significant differences could be observed between both populations with respect to the immunophenotype, expansion capacity and expression of immune inhibitory factors and cell adhesion molecules. Conclusions. Our data show that RBC lysis buffer treatment may be a good alternative to density fractionation, providing a faster, more robust and easier method to purify AT-MSCs with biologically preserved characteristics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据