期刊
CYTOTHERAPY
卷 15, 期 7, 页码 792-804出版社
INFORMA HEALTHCARE
DOI: 10.1016/j.jcyt.2013.04.004
关键词
astrocyte migration; BDNF-overexpressing bone marrow stromal cells; platelet-rich plasma; remyelination; spinal cord injury
类别
资金
- National Natural Science Foundation of China [81171703, 81101377, 81171687, 81271356]
- Natural Science Foundation of Zhejiang, China [Y207216, Y2100161, Y2090283]
- Scientific Research Fund of Zhejiang Provincial Education Department [Y201018936]
- Zhejiang Provincial Department of science and technology [2011C13033]
Background aims. Combining biologic matrices is becoming a better choice to advance stem cell-based therapies. Platelet-rich plasma (PRP) is a biologic product of concentrated platelets and has been used to promote regeneration of peripheral nerves after injury. We examined whether PRP could induce rat bone marrow stromal cells (BMSCs) differentiation in vitro and whether a combination of BMSCs, PRP and brain-derived neurotrophic factor (BDNF) could provide additive therapeutic benefits in vivo after spinal cord injury (SCI). Methods. BMSCs and BDNF-secreting BMSCs (BDNF-BMSCs) were cultured with PRP for 7 days and 21 days, respectively, and neurofilament (NF)-200, glial fibrillary acidic protein (GFAP), microtubule-associated protein 2 (MAP2) and ribosomal protein S6 kinase (p70S6K) gene levels were assessed. After T10 hemi-section in 102 rats, 15-mu L, scaffolds (PRP alone, BMSCs, PRP/BMSCs, BDNF-BMSCs or PRP/BDNF-BMSCs) were transplanted into the lesion area, and real-time polymerase chain reaction, Western blot, immunohistochemistry and ultrastructural studies were performed. Results. The messenger RNA expression of NF-200, GFAP, MAP2 and p7056K was promoted in BMSCs and BDNF-BMSCs after culture with PRP in vitro. BDNF levels were significantly higher in the injured spinal cord after implantation of BDNF-BMSCs. In the PRP/BDNF-BMSCs group at 8 weeks postoperatively, more GFAP was observed, with less accumulation of astrocytes at the graft-host interface. Rats that received PRP and BDNF-BMSC implants showed enhanced hind limb locomotor performance at 8 weeks postoperatively compared with control animals, with more axonal remyelination. Conclusions. A combined treatment comprising PRP and BDNF-overexpressing BMSCs produced beneficial effects in rats with regard to functional recovery after SCI through enhancing migration of astrocytes into the transplants and axonal remyelination.
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