期刊
CYTOTHERAPY
卷 15, 期 10, 页码 1275-1285出版社
INFORMA HEALTHCARE
DOI: 10.1016/j.jcyt.2013.03.009
关键词
adipose-derived stem cells; iron oxide particles; magnetic resonance imaging; peripheral nerve injury; stem cell therapy
类别
资金
- National Natural Science Foundation of China [30872630, 30772104]
- Shanghai Shenkang Laboratory Fund [SHDC12007706]
- Natural Science Foundation of Shanghai Science and Technology Committee [11ZR1429300]
- Medical Guiding Program of Shanghai Science and Technology Committee [1141190800]
- Songjiang Medical Climbing Program, Shanghai, China [2011PD04]
Background aims. Adipose-derived stem cells (ADSCs) have shown great promise in the regenerative repair of injured peripheral nerves. Magnetic resonance imaging (MRI) has provided attractive advantages in tracking superparamagnetic iron oxide nanoparticle (SPION)-labeled cells and evaluating their fate after cell transplantation. This study investigated the feasibility of the use of MRI to noninvasively track ADSCs repair of peripheral nerve injury in vivo. Methods. Green fluorescent protein (GFP)-expressing ADSCs were isolated, expanded, differentiated into an SC-like phenotype (GFP-dADSCs) at early passages and subsequently labeled with SPIONs. The morphological and functional properties of the GFP-dADSCs were assessed through the use of immunohistochemistry. The intracellular stability, proliferation and viability of the labeled cells were evaluated in vitro. Through the use of a microsurgical procedure, the labeled cells were then seeded into sciatic nerve conduits in C57/BL6 mice to repair a 1-cm sciatic nerve gap. A clinical 3-T MRI was performed to investigate the GFP-dADSCs in vitro and the transplanted GFP-dADSCs inside the sciatic nerve conduits in vivo. Results. The GFP-dADSCs were efficiently labeled with SPIONs, without affecting their viability and proliferation. The labeled cells implanted into the mice sciatic nerve conduit exhibited a significant increase in axonal regeneration compared with the empty conduit and could be detected by MRI. Fluorescent microscopic examination, histological analysis and immunohistochemistry confirmed the axon regeneration and MRI results. Conclusions. These data will elucidate the neuroplasticity of ADSCs and provide a new protocol for in vivo tracking of stem cells that are seeded to repair injured peripheral nerves.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据