期刊
CYTOTHERAPY
卷 15, 期 3, 页码 323-329出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jcyt.2012.11.011
关键词
bone marrow; mesenchymal stem cells; species variation; spontaneous calcification
类别
资金
- National Natural Science Foundation [31070872, 31170948]
- National High Technology Research and Development Program of China [2012AA020503]
Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous culture. However, it is unclear whether BM-MSCs from other species share this characteristic. In this study, spontaneous calcification of BM-MSCs from rat, goat, and human specimens was investigated in vitro. BM-MSCs were cultured in complete medium, and calcification was determined by morphologic observation and alizarin red staining. It was demonstrated that rat BM-MSCs possessed a typically spontaneous calcification, whereas goat and human BM-MSCs under the same system proliferated significantly but did not calcify spontaneously. The significant species variation in spontaneous calcification of BM-MSCs described in this study provides useful information regarding evaluation of numerous BM-MSC-based approaches for bone regeneration and the safety of BM-MSCs.
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