期刊
CYTOTHERAPY
卷 11, 期 7, 页码 886-896出版社
ELSEVIER SCI LTD
DOI: 10.3109/14653240903180076
关键词
cell expansion; cell proliferation; cytokines; hematopoietic progenitors; in vitro; mesenchymal stromal cells
类别
资金
- Mexican Institute of Social Security (IMSS, Mexico) [FP-2005/ 2/ I/ 365]
- National Council of Science and Technology (CONACYT, Mexico) [7204]
Background aims We have previously characterized the in vitro growth of two cord blood-derived hematopoietic cell populations in liquid cultures supplemented with recombinant cytokines. In the present study, we assessed the effects of bone marrow-derived mesenchymal stromal cells (MSC) on the growth of such cells. Methods CD34(+) CD38(+) Lin(-) and CD34(+) CD38(-) Lin(-) cells were obtained by negative selection, and cultured in the presence of marrow-derived MSC and/or early-and late-acting cytokines. Hematopoietic cell growth was assessed throughout a 30-day culture period. Results In the presence of MSC alone, both populations showed significant proliferation. Direct contact between MSC and CD34(+) cells was fundamental for optimal growth, especially for CD34(+) CD38(-) Lin(-) cells. In the presence of early-acting cytokines alone, cell growth was significantly higher than in cultures established with MSC but no cytokines. In cultures containing both MSC and early-acting cytokines, a further stimulation was observed only for CD34(+) CD38(-) Lin(-) cells. The cytokine cocktail containing both early-and late-acting cytokines was significantly more potent at inducing hematopoietic cell growth than the early-acting cytokine cocktail. When cultures were supplemented with early-and late-acting cytokines, MSC had no further effect on the growth of hematopoietic cells. Conclusions MSC seem to play a key role, particularly on more primitive (CD34(+) CD38(-) Lin(-)) cells, only in the absence of cytokines or the presence of early-acting cytokines. When both early-and late-acting cytokines are present in culture, MSC seem to be unnecessary for optimal development of CFC and CD34(+) cells.
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