期刊
CYTOTHERAPY
卷 10, 期 8, 页码 796-805出版社
ELSEVIER SCI LTD
DOI: 10.1080/14653240802461924
关键词
experimental diabetes; marrow stromal cells; pigs; subcapsular pancreas; transplantation
类别
资金
- national 973 special plan [2007CB512402]
- Natural Science of Guangdong Province [6027540]
Background Stem cell differentiation is controlled by extracellular cues from the environment and by intrinsic genetic programs within the stem cell. The present study aimed to explore whether mesenchymal stromal cells (MSC) could improve hyperglycemia and insulin production in the diabetic microenvironment. Methods We transplanted male porcine bone marrow-derived EGFP-expressing MSC directly into female diabetic porcine pancreas by multi-point injection. Enzyme-linked immunosorbent assay (ELISA) and fluorescent immunohistochemistry were used to analyze recipients' sera and pancreas tissues for assessment of the therapeutic effect. Results Blood glucose levels decreased gradually in MSC-treated recipients from 15 days after the transplantation compared with untreated diabetic controls (15.94 +/- 0.31 mmol/L versus 16.66 +/- 0.11 mmol/L; P = 0.01). Blood insulin increased and glucagons decreased notably in recipients from 2 weeks post-transplantation compared with untreated diabetic controls (0.049 +/- 0.004 mu g/L versus 0.037 +/- 0.02 mu g/L and 392.9 +/- 20.3 ng/L versus 433.1 +/- 27.6 ng/L). Hematoxylin and eosin (HE)-stained sections demonstrated that the number of islets from each section was markedly increased in recipients compared with that of diabetic controls (10.9 +/- 2.2 versus 4.6 +/- 1.4; P < 0.05) and similar to that of normal controls (10.9 +/- 2.2 versus 12.6 +/- 2.6; P > 0.05). The newly formed islets were smaller than normal islets (47.2 +/- 19.6 mu m versus 119.6 +/- 27.7 mu m; P < 0.05). Analysis of pancreatic sections for EGFP in recipients indicated that the transplanted MSC survived within the pancreas. Insulin immunoreactivity of pancreatic islets showed that the newly formed islets expressed insulin. Discussion: MSC could improve diabetes upon pancreatic microenvironment without obvious immune rejections. This has theoretical and clinical applications.
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