期刊
CYTOTECHNOLOGY
卷 56, 期 3, 页码 161-169出版社
SPRINGER
DOI: 10.1007/s10616-008-9152-1
关键词
hepatopancreas; Scylla serrata; primary cultures; E (Embryonalzellen); F (Fibrenzellen); B (Blasenzellen); R (Restzellen) and G (Granular cells)
This paper reports for the first time, the Primary cell culture of hepatopancreas from edible crab Scylla serrata using crab saline, L-15 (Leibovitz), 1 x L-15 + crab saline, 2 x L-15 + crab saline, 3 x L-15 and citrate buffer without any serum. We could isolate and maintain E (Embryonalzellen), F (Fibrenzellen), B (Blasenzellen), R (Restzellen) and G (Granular cells). Upon seeding the hepatopancreatic E, F, B, and R cells showed different survival pattern over time than granular cells. A modified L-15 (3x) medium supported the best survival of hepatopancreatic E, F B, and R cells in in-vitro culture. However granular cells could be maintained for 184 days with L-15 (1x) + crab saline. Fetal bovine serum was not effective additive and hampered cell viability in present study.
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