期刊
CYTOSKELETON
卷 69, 期 12, 页码 1040-1046出版社
WILEY-BLACKWELL
DOI: 10.1002/cm.21062
关键词
preimplantation embryos; cytokinesis; astral microtubules; spindle positioning
类别
资金
- Agency for Science, Technology and Research (A*STAR) Singapore
Positioning of the cleavage plane is regulated to ensure proper animal development. Most animal cells rely on the astral microtubules to position the mitotic spindle, which in turn specifies the cleavage plane. The mouse zygote lacks discernible astral microtubules but still divides symmetrically. Here, we demonstrate a cloudlike accumulation of F-actin surrounds the spindle in zygotes and when this actin network is disassembled, the spindle assumes an off-center position, and the resulting zygote divides asymmetrically into two unequal size blastomeres. Interestingly, when the spindle is micromanipulated to the subcortical region, the zygote without the actin network is unable to reposition the spindle and cleavage plane at the cell center. This study reveals that an actin network maintains the central spindle position in anastral mitosis, and ensures the first embryonic mitosis is symmetrical. (C) 2012 Wiley Periodicals, Inc
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据