期刊
CYTOMETRY PART B-CLINICAL CYTOMETRY
卷 84, 期 5, 页码 315-323出版社
WILEY-BLACKWELL
DOI: 10.1002/cyto.b.21108
关键词
Cytometry; accuracy; sensitivity; specificity; imprecision
Multi-color flow cytometry is a unique technology, which enables the analysis of heterogeneous cellular systems and provides multiparametric information on a cell-by-cell basis. A variety of factors contribute to the complexity of validating cell-based flow cytometric methods, including the lack of fully characterized cellular reference materials and the difficulty in obtaining, or creating, samples with varying levels of a given cell type or varying levels of expression of a given antigen. This document summarizes validation requirements and describes validation strategies for quasi-quantitative and qualitative cell-based flow cytometric assays. (c) 2013 International Clinical Cytometry Society
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