期刊
CYTOMETRY PART A
卷 85, 期 4, 页码 313-321出版社
WILEY-BLACKWELL
DOI: 10.1002/cyto.a.22400
关键词
phagocytosis; bead uptake; time-resolved; flow cytometry; monocyte
资金
- Nepean Medical Foundation
- Cure Cancer Australia Foundation
- Leukemia Foundation of Australia
- ARC [FT120100581]
- NHMRC Project [1048082]
- Australian Research Council [FT120100581] Funding Source: Australian Research Council
Phagocytosis is central to immunity however a rapid and standardized method is much needed for quantitative assessment of the phagocytic process. We describe a real-time flow cytometric method to quantitate the phagocytosis of fluorescent latex beads by human monocytes in serum-free conditions. Effects of buffer composition, temperature, pH, and bead surface on phagocytic rate are described. The innate phagocytic ability of human monocytes from single subjects measured by this method was relatively stable over many months although phagocytosis rate varied as much as two-fold between individuals. Comparable results were obtained with a simplified method using several mL of whole blood which is suitable for routine clinical application. This method also allows two-color flow cytometric measurement of cytosolic calcium levels during the phagocytic uptake of fluorescent beads. (c) 2013 International Society for Advancement of Cytometry
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据