期刊
CYTOMETRY PART A
卷 75A, 期 4, 页码 371-376出版社
WILEY
DOI: 10.1002/cyto.a.20660
关键词
neurite tracing; NeuronJ; ImageJ; axon guidance; growth cone; netrin; laminin
资金
- NIH [RO1NS049178]
- Fundacao pare a Ciencia e a Tecnologia (FCT), Lisbon, Portugal [PTDC/SAU-NEU/64385/2006, SFRH/BPD/26390/2006]
- FEDER
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/26390/2006] Funding Source: FCT
Studies of neuronal differentiation in vitro often involve tracing and analysis of neurites. Neuron) (Meijering et al., Cytometry Part A 2004;58A:167-176; http://www.imagescience.org/meijering/software/neuronj/) is a program that can be used for semi-automated tracing of individual neurons; when tracing is completed, a text file containing neurite length measurements is generated. Using cultured hippocampal neurons, we have found that to reach statistical significance it is generally necessary to trace about 100 neurons in each treatment group. Posttracing data analysis requires importing each text file into a statistics program. Analysis of distinct parameters, such as effects of a treatment on axonal versus dendritic branching, requires a great deal of time consuming posttracing data manipulation. We have developed XL_Calculations, a Java-based program that performs batch analysis on Neuron) measurement files and automatically makes multiple calculations, including the number, length, and total output (sum length) of primary, secondary, and tertiary neurites on axons and dendrites, and writes the calculations into an Excel worksheet. Batch processing of Neuron) measurement files dramatically reduces the time required to analyze neuronal morphology. In addition, our program performs more than 45 distinct calculations, enabling detailed determination of treatment effects on neuronal differentiation. Using this program to analyze Neuron) tracing data, we demonstrate that continuous exposure of differentiating hippocampal neurons to Netrin 1 increases the number of secondary branches on both axons and dendrites, without significantly altering the length of the axon, dendrites, or branches. Similar results were obtained when neurons were grown on poly-D-lysine or laminin. (C) 2008 International Society far Advancement of Cytometry
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据