期刊
CYTOLOGY AND GENETICS
卷 48, 期 3, 页码 139-144出版社
ALLERTON PRESS INC
DOI: 10.3103/S0095452714030025
关键词
finger millet; genetic transformation; dinitroaniline herbicides; mutant alpha-tubulin
The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAml), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 mu M of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgenic nature of plants. The analysis of seed of T-1 of transgenic lines confirmed heterozygous character of inheritance of trifluralin resistance.
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