Obtaining the Transgenic Lines of Finger Millet Eleusine coracana (L.) Gaertn. with Dinitroaniline Resistance

The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAml), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 mu M of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgenic nature of plants. The analysis of seed of T-1 of transgenic lines confirmed heterozygous character of inheritance of trifluralin resistance.