4.2 Article

Feasibility of [18F]-RGD for ex vivo imaging of atherosclerosis in detection of αvβ3 integrin expression

期刊

JOURNAL OF NUCLEAR CARDIOLOGY
卷 22, 期 6, 页码 1179-1186

出版社

SPRINGER
DOI: 10.1007/s12350-014-0061-8

关键词

MicroPET imaging; vulnerable atherosclerotic plaque; RGD; integrin; imaging; ex vivo

向作者/读者索取更多资源

Background. Inflammation and angiogenesis play an important role in atherosclerotic plaque rupture. Therefore, molecular imaging of these processes could be used for determination of rupture-prone atherosclerotic plaques. alpha v beta 3 integrin is involved in the process of angiogenesis. Targeted imaging of alpha v beta 3 integrin has been shown to be possible in previous studies on tumor models, using radiolabeled arginine-glycine-aspartate (RGD). Our aim was to investigate feasibility of ex vivo detection of alpha v beta 3 integrin in carotid endarterectomy (CEA) specimens. Methods and Results. Nineteen CEA specimens were incubated in 5 MBq [18F]-RGD-K5 for 1 hour followed by 1 hour emission microPET scan. The results were quantified in 4 mm wide segments as percent incubation dose per gram (%Inc/g). Segmental-to-total ratio was calculated and presence of alpha v beta 3 integrin and endothelial cells in each segment was confirmed by immunohistochemical staining for CD31 and alpha v beta 3 integrin, respectively. [18F]-RGD-K5 uptake was heterogeneously distributed across CEA specimens and was localized within the vessel wall. Significant correlations were observed between segmental-to-total ratio with alpha v beta 3 integrin staining score (r = 0.58, P = .038) and CD31 staining score (rho = 0.67, P < .002). Conclusion. This study showed the feasibility of integrin imaging by determination of alpha v beta 3 integrin expression in human atherosclerotic plaques.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.2
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据