期刊
CYTOKINE
卷 45, 期 1, 页码 12-19出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2008.10.006
关键词
inflammatory cytokine; Insulin resistance; The adipose triglyceride lipase(ATGL); Adiponectin; Visfatin
资金
- National Natural Science Foundation of China [30771037, 30871199]
- Chongqing Municipal Education Commission [JK 050304]
- Chongqing medical university [XBZD200704]
- National Institutes of Health [R01-DK 58895]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK058895] Funding Source: NIH RePORTER
inflammatory cytokines have been linked to obesity-related insulin resistance. To investigate the effect of TNF-alpha, an inflammatory cytokine, on insulin action, C57BL/6J mice were treated with TNF-alpha for 7 days after which we examined the in vivo effects of TNF-alpha on glucose tolerance and insulin sensitivity with IV glucose tolerance tests and hyperinsulinemic-euglycemic clamps. In addition, we analyzed the in vivo effect of TNF-alpha on several metabolism-related genes and adipocytokines implicated in the development of insulin resistance. TNF-a treatment resulted in markedly increased fasting blood glucose, insulin and free fatty acids (FFA) levels and reduced glucose tolerance. During the clamps, the rates insulin-stimulated whole body (G(Rd)) and skeletal muscle glucose uptake (MGU) and insulin's ability to suppress hepatic glucose production (HGP) were decreased in TNF-alpha treated animals, indicating insulin resistance. In addition, both PPAR gamma and ATGL mRNA expression in adipose tissues as well as ATGL protein levels in plasma were downregulated. Moreover, adipose mRNA expression and plasma protein levels of adiponectin and visfatin were significantly down-regulated. We conclude that the alterations of PPAR gamma, ATGL, adiponectin and visfatin may contribute to the development of insulin resistance mediated by TNF-alpha. (C) 2008 Elsevier Ltd. All rights reserved.
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