期刊
CYTOGENETIC AND GENOME RESEARCH
卷 141, 期 1, 页码 43-49出版社
KARGER
DOI: 10.1159/000351041
关键词
Arachnid; Ectopic recombination; Facultative heterochromatin; FISH; Meiosis; Spider; 18S rDNA
资金
- Grant Agency of the Academy of Sciences of the Czech Republic [IAA601110808]
- Ministry of Education, Youth and Sports of the Czech Republic [LA10036, SVV 2013-267205]
- Grant Agency of the University of South Bohemia [137/2010/P, 059/2010/P]
In terms of cytogenetics, entelegyne araneomorphs are the best studied clade of spiders. The typical karyotype of entelegyne males consists of acrocentric chromosomes, including 2 non-homologous X chromosomes. The present study is focused on the karyotype, nucleolus organising regions (NORs) and sex chromosome behaviour during meiosis of the entelegyne Wadicosa fidelis (Lycosidae). Preparations stained by Giemsa were used to study karyotype and meiosis. NORs were visualised by silver staining and fluorescence in situ hybridisation with 18S rDNA probe. The male karyotype consists of 28 acrocentric elements, including 2 X chromosomes. In contrast to the majority of other spiders, the male sex chromosomes pair during the major part of meiosis. Following an initial period of parallel pairing, the attachment of male sex chromosomes is restricted to centromeric areas and continues until metaphase II. Our study revealed an enormous number of NORs in the population from Galilee and indicates a considerable variability of NOR numbers in this population. The distal regions of 9 or 10 autosomal pairs contain NORs. The obtained data indicate the rapid spread of NORs in the karyotype of W. fidelis, which was presumably caused by ectopic recombinations and subsequent hybridisations of individuals with different NOR genotypes that produced heterozygotes. Copyright (c) 2013 S. Karger AG, Basel
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