期刊
CURRENT PROTEIN & PEPTIDE SCIENCE
卷 13, 期 6, 页码 585-600出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/138920312803582979
关键词
NMR spectroscopy; membrane protein; single-span membrane protein; structure determination; protein-protein interaction
资金
- Agency for Science, Technology and Research (A*STAR), Singapore
- A*STAR Investigatorship
- A*STAR JCO grant [10/03/FG/06/06]
Membrane proteins play important roles in signal transduction across the cell membrane. Structural information for the membrane proteins is still limited due to many technical challenges. Membrane proteins containing a single alpha-helical transmembrane (TM) domain are very important in several pathways. Solution NMR spectroscopy is an important tool for the study of the structure of the TM domain of these types of proteins due to their small size. In this review, we summarize the importance of some single-span membrane proteins in signal transduction and the importance of understanding the structure of the TM domain. We discussed the current progress in the structural study of these types of proteins using solution NMR spectroscopy. We summarize the structures solved during last several years. The structures of the regulatory domain of the ion channels such as KCNE1, integrin and viral proteins such as the M2 channel are described. The binding interface of single TM-TM domains is discussed based on NMR structural studies. Strategies including sample preparation, detergent screening, and structural determination of single-span membrane protein are summarized. We also discuss the potential application of NMR spectroscopy to drug discovery of proteins with a single-span TM domain.
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