期刊
JOURNAL OF NEUROTRAUMA
卷 32, 期 11, 页码 791-800出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/neu.2014.3564
关键词
axonal injury; controlled cortical impact; intracranial pressure; traumatic brain injury; white matter
资金
- NCRR NIH HHS [S10 RR027552] Funding Source: Medline
- NINDS NIH HHS [R01 NS065069, K08 NS064051] Funding Source: Medline
Reduction and avoidance of increases in intracranial pressure (ICP) after severe traumatic brain injury (TBI) continue to be the mainstays of treatment. Traumatic axonal injury is a major contributor to morbidity after TBI, but it remains unclear whether elevations in ICP influence axonal injury. Here we tested the hypothesis that reduction in elevations in ICP after experimental TBI would result in decreased axonal injury and white matter atrophy in mice. Six-week-old male mice (C57BL/6J) underwent either moderate controlled cortical impact (CCI) (n=48) or Sham surgery (Sham, n=12). Immediately after CCI, injured animals were randomized to a loose fitting plastic cap (Open) or replacement of the previously removed bone flap (Closed). Elevated ICP was observed in Closed animals compared with Open and Sham at 15min (21.4 +/- 4.2 vs. 12.3 +/- 2.9 and 8.8 +/- 1.8mm Hg, p<0.0001) and 1 day (17.8 +/- 3.7 vs. 10.6 +/- 2.0 and 8.9 +/- 1.9mm Hg, p<0.0001) after injury. Beta amyloid precursor protein staining in the corpus callosum and ipsilateral external capsule revealed reduced axonal swellings and bulbs in Open compared with Closed animals (32% decrease, p<0.01 and 40% decrease, p<0.001 at 1 and 7 days post-injury, respectively). Open animals were also found to have decreased neurofilament-200 stained axonal swellings at 7 days post-injury compared with Open animals (32% decrease, p<0.001). At 4 weeks post-injury, Open animals had an 18% reduction in white matter volume compared with 34% in Closed animals (p<0.01). Thus, our results indicate that CCI with decompressive craniectomy was associated with reductions in ICP and reduced pericontusional axonal injury and white matter atrophy. If similar in humans, therapeutic interventions that ameliorate intracranial hypertension may positively influence white matter injury severity.
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