期刊
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
卷 13, 期 14, 页码 2551-2561出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/138920101314151120122527
关键词
Aequorin; bioluminescence; calcium-activated photoprotein; chemiluminescence; coelenteramide; coelenterazine; obelin; oxygenation
资金
- Japan Society for the Promotion of Science [22550031]
- Grants-in-Aid for Scientific Research [22550031] Funding Source: KAKEN
Calcium-activated photoproteins are important and useful bioluminescent reagents for detecting the calcium ion (Ca2+) in biological systems. In conjunction with photon imaging technology, they can be used to observe Ca2+-related life processes in a living cell. To develop useful applications of calcium-activated photoproteins, we need to understand the molecular basis of the bioluminescence reaction. For this purpose, this review describes the oxygenation, chemiexcitation, and light emission processes of calcium-activated photoproteins in the bioluminescence reaction together with the fundamental chemistry of the luminous substrate, coelenterazine, based on recent results from mechanistic chemical studies of these primary processes. Finally, the whole reaction mechanism, including the active site structures of apoproteins, along with available information about the molecular mechanism and the crystallographic structures of calcium-activated photoproteins are summarized.
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