期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 52, 期 -, 页码 64-70出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2018.08.007
关键词
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资金
- European Research Council
- Swiss National Science Foundation
- Gebert Ruf Foundation
- Helmut Horten Foundation
The need for bacteria to interact with their environment has driven the evolution of elaborate secretion systems. By virtue of their function, secretion systems are macromolecular complexes associated with the cell envelope and therefore inherently difficult to study by conventional structural biology techniques. Cryo-electron microscopy (cryoEM) has become an invaluable technique to study large membrane-embedded complexes and led to major advances in the mechanistic understanding of secretion systems. CryoEM comprises of two main modalities, namely single particle analysis and tomography. Here, we review how detailed structures retrieved by single particle analysis combine elegantly with tomography experiments in which the secretion systems are observed in their native cellular context.
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