期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 23, 期 4, 页码 563-568出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2013.04.007
关键词
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资金
- Japan Science and Technology Agency
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Grants-in-Aid for Scientific Research [24121715] Funding Source: KAKEN
Membrane proteins act as gateways to cells, and they are responsible for much of the communication between cells and their environments. Crystallography of membrane proteins is often limited by the difficulty of crystallization in detergent micelles. Co-crystallization with antibody fragments has been reported as a method to facilitate the crystallization of membrane proteins; however, it is widely known that the generation of mouse monoclonal antibodies that recognize the conformational epitopes of mammalian integral membrane proteins is typically difficult. Here, we present our protocols to generate functional mouse antibodies for the membrane protein crystallography, which have enabled us to solve crystal structures of mammalian receptors and transporters complexed with antibody fragments.
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