期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 23, 期 3, 页码 357-364出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2013.03.009
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资金
- Cancer Research, UK
- US National Science Foundation (NSF) [MCB 1157688]
- Showalter Trust Fund
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Centre National pour la Recherche Scientifique (CNRS)
- Association pour la Recherche sur le Cancer (ARC)
- Fondation pour la Recherche Medicale (FRM)
- Agence Nationale de Recherche (ANR)
- European Commission (EC) Framework Programme 7 (FP7) project PCUBE
- European Commission (EC) Framework Programme 7 (FP7) project BioSTRUCT-X
- European Commission (EC) Framework Programme 7 (FP7) project ComplexINC
- European Commission (EC) Framework Programme 7 (FP7) project 4DCellFate
- MRC [MC_UP_1201/6] Funding Source: UKRI
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1157688] Funding Source: National Science Foundation
- Cancer Research UK [14109] Funding Source: researchfish
- Medical Research Council [MC_UP_1201/6] Funding Source: researchfish
Most essential functions in eukaryotic cells are catalyzed by complex molecular machines built of many subunits. To fully understand their biological function in health and disease, it is imperative to study these machines in their entirety. The provision of many essential multiprotein complexes of higher eukaryotes including humans, can be a considerable challenge, as low abundance and heterogeneity often rule out their extraction from native source material. The baculovirus expression vector system (BEVS), specifically tailored for multiprotein complex production, has proven itself to be uniquely suited for overcoming this impeding bottleneck. Here we highlight recent major achievements in multiprotein complex structure research that were catalyzed by this versatile recombinant complex expression tool.
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