期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 23, 期 3, 页码 345-356出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2013.04.003
关键词
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资金
- UK Medical Research Council Career Development Award Fellowship
- MRC research council
- BBSRC research council
- Medical Research Council [MR/L009609/1, G0700232] Funding Source: researchfish
- MRC [G0700232, MR/K018779/1, MR/L009609/1] Funding Source: UKRI
Mammalian cells are rapidly becoming the system of choice for the production of recombinant glycoproteins for structural biology applications. Their use has enabled the structural investigation of a whole new set of targets including large, multi-domain and highly glycosylated eukaryotic cell surface receptors and their supra-molecular assemblies. We summarize the technical advances that have been made in mammalian expression technology and highlight some of the structural insights that have been obtained using these methods. Looking forward, it is clear that mammalian cell expression will provide exciting and unique opportunities for an integrative approach to the structural study of proteins, especially of human origin and medically relevant, by bridging the gap between the purified state. and the cellular context.
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