期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 23, 期 3, 页码 326-334出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2013.01.006
关键词
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资金
- Centre National de la Recherche Scientifique (CNRS)
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Universite Aix-Marseille 2
- Universite de Strasbourg (UDS)
- French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05-01]
Escherichia coli is the major expression host for the production of homogeneous protein samples for structural studies. The introduction of high-throughput technologies in the last decade has further revitalized E. coli expression, with rapid assessment of different expression strategies and successful production of an ever-increasing number of proteins. In addition, miniaturization of biophysical characterizations should soon help choosing expression strategies based on quantitative and qualitative observations. Since many proteins form larger assemblies in vivo, dedicated co-expression systems for E. coli are now addressing the reconstitution of protein complexes. Yet, co-expression approaches show an increasing experimental combinatorial intricacy when considering larger complexes. The current combination of high-throughput and co-expression technologies paves the way, however, for tackling larger and more complex macromolecular assemblies.
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