期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 18, 期 5, 页码 609-616出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2008.06.002
关键词
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Using X-ray absorption spectroscopy (XAS) the binding modes (type and number of ligands, distances and geometry) and oxidation states of metals and other trace elements in crystalline as well as non-crystalline samples can be revealed. The method may be applied to biological systems as a 'standalone' technique, but it is particularly powerful when used alongside other X-ray and spectroscopic techniques and computational approaches. In this review, we highlight how biological XAS is being used in concert with crystallography, spectroscopy and computational chemistry to study metalloproteins in crystals, and report recent applications on relatively rare trace elements utilised by living organisms and metals involved in neurodegenerative diseases.
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