期刊
CURRENT OPINION IN ORGAN TRANSPLANTATION
卷 13, 期 4, 页码 445-451出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MOT.0b013e328303df04
关键词
islet isolation; islet transplants; pancreas preservation; pancreas procurement; pancreas transplants
资金
- NCRR NIH HHS [U42 RR016598-07, U42 RR016598-06S1, U42 RR016598-08, U42 RR016598, U42 RR016598-06, U42 RR016598-05S1] Funding Source: Medline
- NIDDK NIH HHS [R43 DK070400-01A1, R44 DK070400, R43 DK070400-02, R43 DK070400] Funding Source: Medline
Purpose of review To summarize advances and limitations in pancreas procurement and preservation for pancreas and islet transplantation, and review advances in islet protection and preservation. Recent findings Pancreases procured after cardiac death, with in-situ regional organ cooling, have been successfully used for islet transplantation. Colloid-free Celsior and histidine-tryptophanketoglutarate preservation solutions are comparable to University of Wisconsin solution when used for cold storage before pancreas transplantation. Colloid-free preservation solutions are inferior to University of Wisconsin solution for pancreas preservation prior to islet isolation and transplantation. Clinical reports on pancreas and islet transplants suggest that the two-layer method may not offer significant benefits over cold storage with the University of Wisconsin solution: improved oxygenation may depend on the graft size; benefits in experimental models may not translate to human organs. Improvements in islet yield and quality occurred from pancreases treated with inhibitors of stress-induced apoptosis during procurement, storage, isolation or culture. Pancreas perfusion may be desirable before islet isolation and transplantation and may improve islet yields and quality. Methods for real-time, wnoninvasive assessment of pancreas quality during preservation have been implemented and objective islet potency assays have been developed and validated. These innovations should contribute to objective evaluation and establishment of improved pancreas preservation and islet isolation strategies. Summary Cold storage may be adequate for preservation before pancreas transplants, but insufficient when pancreases are processed for islets or when expanded donors are used. Supplementation of cold storage solutions with cytoprotective agents and perfusion may improve pancreas and islet transplant outcomes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据