期刊
JOURNAL OF NEUROSCIENCE
卷 35, 期 38, 页码 13206-13218出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0193-15.2015
关键词
A-type K+ channels; action potential repolarization; aging; CA3; Kv4.2/Kv4.3; pyramidal neurons
资金
- National Institutes of Health [AG08796, AG017139, AG047073]
- Charles and M.R. Shapiro Foundation
- Schild Fund
Aging-related impairments in hippocampus-dependent cognition have been attributed to maladaptive changes in the functional properties of pyramidal neurons within the hippocampal subregions. Much evidence has come from work on CA1 pyramidal neurons, with CA3 pyramidal neurons receiving comparatively less attention despite its age-related hyperactivation being postulated to interfere with spatial processing in the hippocampal circuit. Here, we use whole-cell current-clamp to demonstrate that aged rat (29-32 months) CA3 pyramidal neurons fire significantly more action potentials (APs) during theta-burst frequency stimulation and that this is associated with faster AP repolarization (i.e., narrower AP half-widths and enlarged fast afterhyperpolarization). Using a combination of patch-clamp physiology, pharmacology, Western blot analyses, immunohistochemistry, and array tomography, we demonstrate that these faster AP kinetics are mediated by enhanced function and expression of Kv4.2/Kv4.3 A-type K+ channels, particularly within the perisomatic compartment, of CA3 pyramidal neurons. Thus, our study indicates that inhibition of these A-type K+ channels can restore the intrinsic excitability properties of aged CA3 pyramidal neurons to a young-like state.
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