期刊
JOURNAL OF NEUROSCIENCE
卷 35, 期 25, 页码 9369-9380出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5054-14.2015
关键词
auditory; GABA; GABA(A)R; thalamus; tinnitus
资金
- Office of Naval Research [N00014-12-1-0214]
- National Institute on Deafness and Other Communication Disorders [DC-000151]
Accumulating evidence suggests a role for inhibitory neurotransmitter dysfunction in the pathology of tinnitus. Opposing hypotheses proposed either a pathologic decrease or increase of GABAergic inhibition in medial geniculate body (MGB). In thalamus, GABAmediates fast synaptic inhibition via synaptic GABA(A) receptors (GABA(A)Rs) and persistent tonic inhibition via high-affinity extrasynaptic GABA(A)Rs. Given that extrasynaptic GABA(A)Rs control the firing mode of thalamocortical neurons, we examined tonic GABA(A)R currents in MGB neurons in vitro, using the following three groups of adult rats: unexposed control (Ctrl); sound exposed with behavioral evidence of tinnitus (Tin); and sound exposed with no behavioral evidence of tinnitus (Non-T). Tonic GABA(A)R currents were evoked using the selective agonist gaboxadol. Months after a tinnitus-inducing sound exposure, gaboxadol-evoked tonic GABA(A)R currents showed significant tinnitus-related increases contralateral to the sound exposure. In situ hybridization studies found increased mRNA levels for GABA(A)R delta-subunits contralateral to the sound exposure. Tin rats showed significant increases in the number of spikes per burst evoked using suprathreshold-injected current steps. In summary, we found little evidence of tinnitus-related decreases in GABAergic neurotransmission. Tinnitus and chronic pain may reflect thalamocortical dysrhythmia, which results from abnormal theta-range resonant interactions between thalamus and cortex, due to neuronal hyperpolarization and the initiation of low-threshold calcium spike bursts (Walton and Llinas, 2010). In agreement with this hypothesis, we found tinnitus-related increases in tonic extrasynaptic GABA(A)R currents, in action potentials/evoked bursts, and in GABA(A)R delta-subunit gene expression. These tinnitus-related changes in GABAergic function may be markers for tinnitus pathology in the MGB.
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