期刊
ACS NANO
卷 9, 期 3, 页码 2565-2573出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn5057595
关键词
multivalent network; point of care; phospholipase; biosensor; lateral flow device
类别
资金
- Technology Strategy Board (TSB)
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/K502352/1]
- ERC-POC grant Naturale-POC
- ERC consolidator grant Naturale-CG
- Engineering and Physical Sciences Research Council [EP/K502352/1] Funding Source: researchfish
- EPSRC [EP/K502352/1] Funding Source: UKRI
A rapid and highly sensitive point-of-care (PoC) lateral flow assay for phospholipase A(2) (PLA(2)) is demonstrated in serum through the enzyme-triggered release of a new class of biotinylated multiarmed polymers from a liposome substrate. Signal from the enzyme activity is generated by the adhesion of polystreptavidin-coated gold nanoparticle networks to the lateral flow device, which leads to the appearance of a red test line due to the localized surface plasmon resonance effect of the gold. The use of a liposome as the enzyme substrate and multivalent linkers to link the nanoparticles leads to amplification of the signal, as the cleavage of a small amount of lipids is able to re-lease a large amount of polymer linker and adhesion of an even larger amount of gold nanoparticles. By optimizing the molecular weight and multivalency of these biotinylated polymer linkers, the sensitivity of the device can be tuned to enable naked-eye detection of 1 nM human PLA(2) in serum within 10 min. This high sensitivity enabled the correct diagnosis of pancreatitis in diseased clinical samples against a set of healthy controls using PLA(2) activity in a point-of-care device for the first time.
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