期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 16, 期 3-4, 页码 436-443出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2012.05.181
关键词
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Magnetic particles can combine two very selective processes in bioanalysis: the specific binding of analytes to the particle surface based on molecular recognition and the specific isolation of magnetic objects from complex sample mixtures. They have found numerous applications including cell isolation, immunoassays or DNA extraction. In this review recent trends in the use of magnetic particles are presented. Integrated sample-in-answer-out lab-on-a-chip systems often employ magnetic particles for at least one of the reaction steps. Several groups have shown on-particle processing in continuous flow for assays and DNA extractions. Other researchers have demonstrated the manoeuvring and splitting of magnetically functionalised droplets for various bioapplications. Improvements in magnet configuration now allow for sorting of magnetically labelled cells within mL volumes in minutes.
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