期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 15, 期 6, 页码 831-837出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2011.10.005
关键词
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Imaging contrasts other than fluorescence are highly desirable for label-free detection and interrogation of nonfluorescent molecular species inside live cells, tissues, and organisms. The recently developed stimulated Raman scattering (SRS) and stimulated emission microscopy techniques provide sensitive and specific contrast mechanisms for nonfluorescent species, by employing the light amplification aspect of stimulated radiation. Compared to their spontaneous counterparts, stimulated radiation can enhance the imaging performance significantly, making the previously 'dark' molecules observable. Here we review and summarize the underlying principles of this emerging class of molecular imaging techniques.
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