期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 15, 期 6, 页码 853-863出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.cbpa.2011.11.002
关键词
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资金
- Burroughs Wellcome Fund CABS [CABS 1004856]
- NSF [MCB 0644262]
- NIH-NIGMS [R01 GM084288]
- American Cancer Society [RSG GMC-117152]
- Columbia University's NSF
Combined with the availability of highly purified, fluorescently labeled in vitro translation systems, the advent of single-molecule fluorescence imaging has ushered in a new era in high-resolution mechanistic studies of ribosome-catalyzed protein synthesis, or translation. Together with ensemble biochemical investigations of translation and structural studies of functional ribosomal complexes, in vitro single-molecule fluorescence imaging of protein synthesis is providing unique mechanistic insight into this fundamental biological process. More recently, rapidly evolving breakthroughs in fluorescence-based molecular imaging in live cells with sub-diffraction-limit spatial resolution and ever-increasing temporal resolution provide great promise for conducting mechanistic studies of translation and its regulation in living cells. Here we review the remarkable recent progress that has been made in these fields, highlight important mechanistic insights that have been gleaned from these studies thus far, and discuss what we envision lies ahead as these approaches continue to evolve and expand to address increasingly complex mechanistic and regulatory aspects of translation.
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