期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 13, 期 1, 页码 3-9出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2009.01.017
关键词
-
资金
- Ruth M Kirschstein National Research Service [F32 GM076964]
- NIH [R01 GM074712-01A1]
- Institute for Collaborative Biotechnologies
- Jacobs Institute for Molecular Medicine
- Department of Energy
Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse. Activities on new substrates can be obtained by improving variants with broadened specificities or by step-wise evolution through a sequence of more and more challenging substrates. Evolution of highly specific enzymes has been demonstrated, even with positive selection alone. It is apparent that many solutions exist for any given problem, and there are often many paths that lead uphill, one step at a time.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据