期刊
JOURNAL OF NEUROSCIENCE
卷 35, 期 9, 页码 3893-3902出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4402-14.2015
关键词
branching; calcium; Coronin-1; NGF; PI3K; TrkA
资金
- National Institutes of Health (NIH)-National Institute of Neurological Disorders and Stroke (NINDS) [R01NS083265]
- Sloan Foundation
- UVa Fund for Excellence in Science and Technology
- NIH-NINDS [1R01NS072388]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1305808] Funding Source: National Science Foundation
Development of a functional peripheral nervous system requires axons to rapidly innervate and arborize into final target organs and then slow but not halt their growth to establish stable connections while keeping pace with organ growth. Here we examine the role of the NGF-TrkA effector protein, Coronin-1, on postganglionic sympathetic neuron final target innervation. In the absence of Coronin-1 we find that NGF-TrkA-PI3K signaling drives robust axon growth and branching in part by suppressing GSK3 beta. In contrast, the presence of Coronin-1 (wild-type neurons) suppresses but does not halt NGF-TrkA-dependent growth and branching. This relative suppression in axon growth behaviors is due to Coronin-1-dependent calcium release via PLC-gamma 1 signaling, which releases PI3K-dependent suppression of GSK3 beta. Finally, we demonstrate that Coro1a(-/-) mice display sympathetic axon overgrowth and overbranching phenotypes in the developing heart. Together with previous work demonstrating the Coronin-1 expression is NGF dependent, this work suggests that periods before and after NGF-TrkA-induced Coronin-1 expression (and likely other factors) defines two distinct axon growth states, which are critical for proper circuit formation in the sympathetic nervous system.
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