期刊
CURRENT OPINION IN CELL BIOLOGY
卷 29, 期 -, 页码 82-91出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.ceb.2014.04.008
关键词
-
类别
资金
- JSPS KAKENHI Grant [25870312, 25291023]
- MEXT KAKENHI Grant [24112006]
- National Institutes of Health [GM75061, DK79307]
- Grants-in-Aid for Scientific Research [24112006, 25870312] Funding Source: KAKEN
Endoplasmic reticulum-associated degradation (ERAD) is a mechanism during which native and misfolded proteins are recognized and retrotranslocated across the ER membrane to the cytosol for degradation by the ubiquitin-proteasome system. Like other cellular pathways, the factors required for ERAD have been analyzed using both conventional genetic and biochemical approaches. More recently, however, an integrated top-down approach has identified a functional network that underlies the ERAD system. In turn, bottom-up reconstitution has become increasingly sophisticated and elucidated the molecular mechanisms underlying substrate recognition, ubiquitylation, retrotranslocation, and degradation. In addition, a live cell imaging technique and a site-specific in vivo photo-crosslinking approach have further dissected specific steps during ERAD. These technical developments have revealed an unexpected dynamicity of the membrane-associated ERAD complex. In this article, we will discuss how these technical developments have improved our understanding of the ERAD pathway and have led to new questions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据