期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 24, 期 1, 页码 88-94出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2012.09.008
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资金
- Deutsche Forschungsgemeinschaft (DFG) [INST 268/149-1]
Estimation of growth potential in a complex community is a great challenge in biotechnical processes and environmental water quality control. Recently it has been shown that the macromolecular structure is a good indicator for the growth potential of phytoplankton cells. A functional understanding of natural phytoplankton communities requires a community analysis by means of single particles technologies. However, conventional biochemical methods are not sensitive enough to determine the macromolecular composition of a single cell or cell aggregates. This problem can be resolved by Fourier transform infrared (FIR) spectroscopy, which delivers results similar to biochemical analysis with a much smaller sample size. The combined approach of flow cytometric analysis with subcommunity sorting and subsequent FTIR-analysis offers new perspectives for the understanding of community functioning and process optimization.
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