期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 24, 期 6, 页码 979-986出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2013.03.024
关键词
-
Metabolic flux analysis (MFA) using isotopic tracers aims at the experimental determination of in vivo reaction rates (fluxes). In recent years, the well-established C-13-MFA method based on metabolic and isotopic steady state was extended to INST-MFA (isotopically non-stationary MFA), which is performed in a transient labeling state. INST-MFA offers short-time experiments with a maximal information gain, and can moreover be applied to a wider range of growth conditions or organisms. Some of these conditions are not accessible by conventional methods. This comes at the price of significant methodological complexity involving high-frequency sampling and quenching, precise analysis of many samples and an extraordinary computational effort. This review gives a brief overview of basic principles, experimental workflows, and recent progress in this field. Special emphasis is laid on the trade-off between total effort and information gain, particularly on the suitability of INST-MFA for certain types of biological questions. In order to integrate INST-MFA as a viable method into the toolbox of MFA, some major challenges must be addressed in the coming years. These are discussed in the outlook.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据