期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 20, 期 1, 页码 54-62出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2009.02.008
关键词
-
资金
- DFG [FR1155/8-1]
- EU [LSHC-CT-2004-503569]
- National Instututes of Health [CA103563]
Multiphoton microscopy (MPM) is the method of choice for investigating cells and cellular functions in deep tissue sections and organs. Here we present the setup and applications of infrared-(IR-)MPM using excitation wavelengths above 1080 nm. IR-MPM enables the use of red fluorophores and fluorescent proteins, doubles imaging depth, improves second harmonic generation of tissue structures, and strongly reduces phototoxicity and photobleaching, compared with conventional MPM. Furthermore, it still provides subcellular resolution at depths of several hundred micrometers and thus will enhance long-term live cell and deep tissue microscopy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据