期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 113, 期 9, 页码 3172-3185出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00113.2015
关键词
sodium channel; Na(v)1.8; persistent current; ramp current; dorsal root ganglion
资金
- Medical Research Service, Department of Veterans Affairs
- Rehabilitation Research Service
Although species-specific differences in ion channel properties are well-documented, little has been known about the properties of the human Na(v)1.8 channel, an important contributor to pain signaling. Here we show, using techniques that include voltage clamp, current clamp, and dynamic clamp in dorsal root ganglion (DRG) neurons, that human Na(v)1.8 channels display slower inactivation kinetics and produce larger persistent current and ramp current than previously reported in other species. DRG neurons expressing human Na(v)1.8 channels unexpectedly produce significantly longer-lasting action potentials, including action potentials with half-widths in some cells >10 ms, and increased firing frequency compared with the narrower and usually single action potentials generated by DRG neurons expressing rat Na(v)1.8 channels. We also show that native human DRG neurons recapitulate these properties of Na(v)1.8 current and the long-lasting action potentials. Together, our results demonstrate strikingly distinct properties of human Na(v)1.8, which contribute to the firing properties of human DRG neurons.
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