期刊
CURRENT MOLECULAR MEDICINE
卷 14, 期 3, 页码 370-375出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1566524014666140228100250
关键词
microRNA; muscle fiber; MyoD; regulation; Siniperca chuatsi
资金
- National Natural Science Foundation of China [31230076, 31340054]
- Natural Science Foundation of Hunan province [14JJ2135, 14JJ3137]
- State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB01]
The fast and slow skeletal muscle fibers in fish are separated to a much greater degree than in mammals. MyoD is required for the maintenance of normal fiber type balance in muscles. So far, the upstream regulatory factors of MyoD in terms of controlling muscle phenotype are poorly understood. In the present study, we used Siniperca chuatsi as a model system and demonstrated that miR-143 expression was negatively correlated with MyoD expression in the fast and slow muscles of S. chuatsi. The luciferase reporter assay further verified the direct interaction between the miR-143 and MyoD. The miR-143 suppression also led to the significant increase in MyoD and fast myosin heavy chain gene expression in vivo. Taken together, our studies indicated that miRNA participates in controlling the performance of different muscle fiber types in vertebrates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据